Fig. 1

Composition of the p1 gene and sequence alignments of primer binding sites. A The illustration of the p1 gene indicating the RepMP4 and RepMP2/3 regions; B Sequence alignments of type 1 and 2 primer and molecular beacon binding sites. Sequence alignment picture was drawn using the tool reported [38]. Blue solid lines were used to indicate binding sequences of type 1 primer and molecular beacon. Green dotted lines were used to indicate binding sequences of type 2 primer and molecular beacon