Clinical performance of quantitative PCR for the molecular identification of skeletal tuberculosis from formalin-fixed paraffin-embedded tissues

Table 3 Detection performance of qPCR and AFS in various conditions

	TP	TN	FP	FN	Sensitivity (%)	p-value	Specificity (%)	p-value
Decalcified group
qPCR	24	22	0	27	47.05		100
AFS	13	22	2	38	25.49	0.023	91.67	0.166
Non-decalcified group
qPCR	20	17	0	19	51.28		100
AFS	21	17	8	18	53.85	0.821	68	0.01
Spinal TB
qPCR	38	24	0	41	48.10		100
AFS	31	24	8	48	39.24	0.262	72.2	0.008
Extraspinal TB
qPCR	6	15	0	5	54.45		100
AFS	3	15	2	8	27.24	0.139	88.2	0.170
Skeletal and pulmonary TB
qPCR	18	2	0	3	85.71		100
AFS	11	2	0	10	52.3	0.019	100	0.301
Skeletal TB alone
qPCR	26	37	0	43	52.3		100
AFS	22	37	10	47	31.88	0.475	78.7	0.003

TP true positive, FN false negative, TN true negative, FP false positive, PPV positive predictive value, NPV negative predictive value. The Chi-squared test was used for the statistical analyses

ISSN: 1471-2334