Fig. 5

The bloodstream isolates KPN49 and KPN165 harbor KP52.145pII-like plasmids. De novo clustering of plasmids using Mash (A). Individual nodes represent a sequenced plasmid. The thickness of the edge connecting plasmids is determined by Jaccard index as indicated. Networks were graphed using Cytoscape. The indicated plasmid sequences were aligned to pKPN49_1 using BRIG (B). A sequence identity threshold of 85% was used. The ~ 40 kb tra locus insertion is labeled. Aerobactin biosynthesis genes are indicated with green arrows, salmochelin with orange arrows, and rmpA with a blue arrow. Plasmid accession numbers for previously published plasmids are listed in Table S7