Table 2 Assay performance of phage DNA detection-based Taqman qPCR and gltA-based Taqman qPCR for spiked clinical swab and bronchoalveolar fluid samples
LB8 | Taqman/gltA | Phage DNA detection-based Taqman qPCR | ||||||
|---|---|---|---|---|---|---|---|---|
Simulate samples | Swab | Bronchoalveolar fluid | Swab | Bronchoalveolar fluid | Phage | |||
Time(h) | 0 | 3 | 0 | 3 | 3 | 3 | 0 | |
9 | 23.6 ± 0.5 | 22 ± 0.1 | 25.2 ± 0.6 | 22.5 ± 1.0 | 22 | 14.7 ± 0.4 | 31.2 ± 0.4 | |
△Ct | 9.2 | 16.5 | ||||||
6 | 32.7 ± 0.9 | 28.5 ± 0.3 | 30.3 ± 0.7 | 29.3 ± 0.6 | 28.1 ± 0.4 | 25.5 ± 1.2 | ||
△Ct | 3.1 | 5.6 | ||||||
3 | U | 36.1 ± 0.9 | U | 35.5 ± 1.5 | 26.5 ± 1.7 | 29.2 ± 0.1 | ||
△Ct | 4.7 | 2.0 | ||||||
1 | U | U | U | U | 29.8 ± 0.8 | 30.4 ± 1.2 | ||
△Ct | 1.4 | 0.8 | ||||||