Table 5 ROC curve analysis of two step RT-qPCR a and one-step RT-qPCR Ct
From: Diagnostic power of one-step and two-step RT-qPCR methods to SARS‑CoV‑2 detection
Target | Method | AUC (95% CI) | Sensitivity (%) (95% CI) | Specificity (%) (95% CI) | Cut-off value | P-value |
|---|---|---|---|---|---|---|
S gene | Two step RT-qPCR | 0.90 (0.79–0.97) | 88 (68.8–97.5) | 88 (68.8–97.5) | ≤ 40 | 0.0471 |
N gene | Two step RT-qPCR | 0.79 (0.66–0.89) | 88 (68.8–97.5) | 84 (63.9–95.5) | ≤ 40 | 0.0489 |
RdRp gene | One step RT-qPCR | 0.95 (0.86–0.99) | 92 (74.0–99) | 80 (59.3–93.2) | ≤ 40 | 0.0234 |
N gene | One step RT-qPCR | 0.95 (0.86–0.99) | 92 (74.0–99) | 96 (79.6–99.9) | ≤ 40 | 0.0356 |
S and N gene | Two step RT-qPCR | 0.85 (0.72–0.93) | 88 (68.8–97.5) | 86 (66.3–96.5) | ≤ 40 | 0.0461 |
RdRp and N gene | One step RT-qPCR | 0.95 (0.86–0.99) | 92 (74.45–99.8) | 88 (69.4–96.55) | ≤ 40 | 0.0441 |