Table 4 Clinical performance of the compared one-step and two-step RT-qPCR for diagnosis SARS-CoV-2
From: Diagnostic power of one-step and two-step RT-qPCR methods to SARS‑CoV‑2 detection
Method | Target | Accuracy (%) | PPV (%) | NPV (%) |
|---|---|---|---|---|
Two step RT-qPCR (SYBR Green-based) | S gene | 88 | 85 | 91 |
N gene | 86 | 84 | 87 | |
One step RT-qPCR (Prob-based) | RdRp gene | 86 | 82 | 90 |
N gene | 94 | 92 | 95 |