Table 1 Sequence of primers designed for two step RT-qPCR method
From: Diagnostic power of one-step and two-step RT-qPCR methods to SARS‑CoV‑2 detection
Gene | Primer | Primer length (bp) | Product length |
|---|---|---|---|
S | F 5ʹ-TCCATCAAAACCAAGCAAGA-3ʹ | 20 | 245 bp |
R 5ʹ-CACCAAAGGTCCAACCAGAA-3ʹ | 20 | NC_045512v2:23,986 + 24,230 | |
N | F 5ʹ-CTGGACTTCCCTATGGTGCT-3ʹ | 20 | 284 bp |
R 5ʹ-ATTGCCAGCCATTCTAGCAG-3ʹ | 20 | NC_045512v2:28,629 + 28,912 |