Skip to main content
Fig. 2 | BMC Infectious Diseases

Fig. 2

From: SEB genotyping: SmartAmp-Eprimer binary code genotyping for complex, highly variable targets applied to HBV

Fig. 2

HBV genotyping detection. a plasmid DNA. Proof of concept using plasmid DNA of HBV full S-region, designed using each of the 8 genotypes consensus sequence (A-H), n = 2. Red sigmoid amplification curves are read as “On” positive signal and no amplification are read as “Off” negative signal. Black curves are no template negative controls (NC). b viral DNA from cell culture supernatant. First validation using viral DNA purified from HBP25 (genotype C) or HepG2.2.15.7 (genotype D) cell culture supernatant, n = 4. c Human serum samples. Nine human serum samples were genotyped with SEB Genotyping, n = 2. + : “On”, positive signal. Δ: Discordant result between the expected negative and the observed positive fluorescence signal detected

Back to article page