Table 1 Primer sets used to determine the mRNA expression levels of TLRs by Real-time PCR
Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|
TLR1 | GAAGATTTCTTGCCACCCTAC | GAACACAATGTGCAGACTCTC |
TLR2 | CTGGACAATGCCACATAC | CTAATGTAGGTGATCCTG |
TLR4 | CAGAACTGCAGGTGCTGG | GTTCTCTAGAGATGCTAG |
TLR6 | CTATTGTTAAAAGCTTCCATTTTGT | ACCTGAAGCTCAGCGATGTAGTTC |
Β-Actin | ACTTAGTTGCGTTACACCCTT | GTCACCTTCACCGTTCCA |