Table 4 HBV DNA and HBsAg level in nails mixed with cell dissociation reagent and PBS with time
From: Signature of chronic hepatitis B virus infection in nails and hair
| Â | Incubation time | Patient A | Patient B | Patient C | Patient D | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HBV DNA | HBsAg | β-actin | HBV DNA | HBsAg | β-actin | HBV DNA | HBsAg | β-actin | HBV DNA | HBsAg | β-actin | ||
log copies/mL | IU/mL | log copies/mL | log copies/mL | IU/mL | log copies/mL | log copies/mL | IU/mL | log copies/mL | log copies/mL | IU/mL | log copies/mL | ||
Cell dissociation reagent | 0Â h | ND | ND | 3.9 | ND | ND | 3.6 | ND | ND | 4 | ND | 0.023 | 3.3 |
1Â h | 3.7 | 0.023 | 5.5 | 3.6 | ND | 4.8 | 3.6 | 0.031 | 5.8 | 2.9 | 0.047 | 5.5 | |
3Â h | 3.8 | 0.034 | 5.7 | 3.8 | 0.023 | 5 | 3.4 | 0.025 | 6 | 3.4 | 0.137 | 5.8 | |
6Â h | 4.1 | 0.032 | 5.8 | 3.9 | 0.052 | 5.3 | 3.1 | 0.024 | 6 | 3.6 | 0.296 | 5.8 | |
12Â h | 3.9 | 0.033 | 5.7 | 4.3 | 0.136 | 5.3 | 2.8 | 0.023 | 6 | 3.8 | 0.798 | 6 | |
24Â h | 4 | 0.028 | 5.7 | 4.6 | 0.402 | 5.7 | 3.1 | 0.025 | 6 | 4.5 | 1.16 | 6.3 | |
Day 3 | 4 | 0.03 | 5.8 | 5 | 0.864 | 6 | 3.2 | 0.025 | 6 | 4.5 | 1.56 | 6.5 | |
Day 5 | 3.9 | 0.045 | 5.7 | 5.1 | 1.12 | 6.3 | 3.3 | 0.022 | 6 | 4.5 | 1.54 | 6.6 | |
Day 7 | 3.8 | 0.048 | 5.7 | 5.2 | 1.32 | 6.3 | 3.2 | 0.021 | 6 | 4.5 | 1.33 | 6.6 | |
PBS | 0Â h | ND | ND | ND | ND | ND | ND | ND | ND | 5.7 | ND | ND | ND |
1Â h | 3.3 | ND | 4.3 | ND | ND | ND | ND | ND | 5 | ND | ND | 4.3 | |
3Â h | 3.8 | ND | 4.8 | ND | ND | ND | ND | ND | 4.9 | ND | ND | 4.3 | |
6Â h | 3.3 | ND | 4 | ND | ND | 3.5 | 2.8 | ND | 4.7 | ND | ND | 4.3 | |
12Â h | 3.4 | ND | 4.6 | ND | ND | 3.8 | ND | ND | 4.6 | ND | ND | 4.7 | |
24Â h | 3.8 | ND | 4.6 | ND | ND | 3.7 | ND | ND | 4.5 | ND | ND | 4.5 | |
Day 3 | 4 | ND | 4.8 | ND | ND | 3.7 | ND | ND | 4.6 | 3 | ND | 4.9 | |
Day 5 | 4.1 | ND | 5 | ND | ND | 4.3 | ND | ND | 4.8 | 3.7 | 0.02 | 4.8 | |
Day 7 | 4.8 | ND | 6 | ND | ND | 4.8 | 3.6 | ND | 4.5 | 4.8 | 0.1 | 5.5 | |