Table 1 Oligonucleotides used as primers to loop-mediated-isothermally amplify the MetA target of N. meningitidis
Oligo names/concentration | Sequences (5′–3′) | Volume uses for one reaction |
|---|---|---|
Tr-Hien-metA-F3(10 pmol/μl) | GCAGTTCCTAATTTACCATGA | 0.5 µl 0.5 µl |
Tr-Hien-metA-B3(10 pmol/μl) | GCAACGAAAATTGCAACTGTA | |
Tr-Hien-metA-FIP(40 pmol/μl) | GGTGAATTTGTTCCCATTATTGCGCACCATGATACCCCCATG | 0.75 µl 0.75 µl |
Tr-Hien-metA-BIP(40 pmol/μl) | TTCACATTTTGGCTGTCAAAGGCTATGATGATTACACCTGT | |
Tr-Hien-metA-LF(10 pmol/μl) | GCTGCTTTTGGCGGTGCATT | 1 µl 1 µl |
Tr-Hien-metA-LB(10 pmol/μl) | CTTGGCTGTCTAAATTTTGCGC | |
TR-H-VapA-NM-gRNA | UAAUUUCUACUAAGUGUAGAUAGCCUGUGAUAAUUGAAUUGC |