Fig. 5From: The first case report of thorn-induced Alternaria alternata infection of the hand in an immunocompetent host Phylogenetic tree constructed based on internal transcribed spacer (ITS) gene sequence of Alternaria alternata. Gene sequencing analysis of ITS gene, amplified with the primer pair ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′TCCTCCGCTTATTGATATGC)-3′, indicated Alternaria alternata. Alternaria alternata was confirmed with an 100% accuracy using the basic local alignment search tool algorithm. (*, patient). Scale bar indicated nucleotide substitutions per siteBack to article page