Table 5 Primer sequence in the RT-PCR
From: Revealing potential diagnostic gene biomarkers of septic shock based on machine learning analysis
Primer name | Primer sequence (5ʹ to 3ʹ) |
|---|---|
GAPDH-F (Internal reference) | 5-CTGGGCTACACTGAGCACC-3 |
GAPDH-R (Internal reference) | 5-AAGTGGTCGTTGAGGGCAATG-3 |
ACTB-F (Internal reference) | 5-TCCGCAAAGACCTGTACGC-3 |
ACTB-R (Internal reference) | 5-CTGGAAGGTGGACAGCGAG-3 |
ARHGEF18-F | 5-ACGCCAGCAAAGAAGACGT-3 |
ARHGEF18-R | 5-CAGGCGGTCATCAGTGGTT-3 |
CLEC5A-F | 5-GCAATTGTCAACACGCCAGA-3 |
CLEC5A-R | 5-GCCAATGGTCGCACAGTTG-3 |
DUSP3-F | 5-GAAGATATGGGGCAACTGGA-3 |
DUSP3-R | 5-ATGCACGTGTTCAGCTTGAG-3 |
FCER1A-F | 5-GTTCTTCGCTCCAGATGGC-3 |
FCER1A-R | 5-TTGTGGAACCATTTGGTGGAA-3 |
HDAC4-F | 5-AGCGTCCGTTGGATGTCAC-3 |
HDAC4-R | 5-CCTTCTCGTGCCACAAGTCT-3 |
KLRF1-F | 5-GGGAATATCTGGAACCGTGA-3 |
KLRF1-R | 5-CTGCAGATCTCGAAGCACAA-3 |
UPP1-F | 5-TGGAGTCCTCGGTGTTTGC-3 |
UPP1-R | 5-GCTCAGGCCTTGCTCAGTT-3 |