Fig. 2From: Rv3737 is required for Mycobacterium tuberculosis growth in vitro and in vivo and correlates with bacterial load and disease severity in human tuberculosis Sequence alignment of Rv3737 sequences in Mtb and Determination of free threonine concentration. A Searched the protein database at the National Center for Biotechnology Information for Rv3737 in Mtb and ThrE in C. glutamicum amino acid sequences. ClustalW software (http://www.clustal.org/) was used to align sequences and reconstruct a phylogenetic tree based on sequence similarities. Shared amino acids are highlighted in red selected for testing in this study. B The free threonine concentration of H37Rv-WT and H37RvΔRv3737 in 7H9 medium grown for 16 days. C The free threonine concentration of Msm/pMV261 and Msm/pMV261-Rv3737 in 7H9 medium grown for 4 days. The difference between the wild type and the mutant was significant by Student’s t test (*p < 0.05; **p < 0.01; ***p < 0.001)Back to article page