Fig. 1From: Rv3737 is required for Mycobacterium tuberculosis growth in vitro and in vivo and correlates with bacterial load and disease severity in human tuberculosisConstruction the Rv3737 knockout Mtb strain and Rv3737 Overexpression Msm strain. A The wildtype Rv3737 was substituted with DNA fragment encoding hygromycin resistance by homologous recombination. B Verification of the Rv3737 knockout Mtb strain by PCR amplification; M: DNA Marker 5000 bps; Lane 2: H37RvΔRv3737 left arm PCR product band; Lane 4: H37RvΔRv3737 right arm PCR product band; H37Rv-WT both arms PCR products have no bands. C Verification of the Rv3737 knockout Mtb strain by qPCR, t test, *p < 0.01. D Verification of the Rv3737 Overexpression Msm strain by Western blotting (full length gels and blots was provided in Additional file 1: Fig. S1 with changes marked)Back to article page