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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Rv3737 is required for Mycobacterium tuberculosis growth in vitro and in vivo and correlates with bacterial load and disease severity in human tuberculosis

Fig. 1

Construction the Rv3737 knockout Mtb strain and Rv3737 Overexpression Msm strain. A The wildtype Rv3737 was substituted with DNA fragment encoding hygromycin resistance by homologous recombination. B Verification of the Rv3737 knockout Mtb strain by PCR amplification; M: DNA Marker 5000 bps; Lane 2: H37RvΔRv3737 left arm PCR product band; Lane 4: H37RvΔRv3737 right arm PCR product band; H37Rv-WT both arms PCR products have no bands. C Verification of the Rv3737 knockout Mtb strain by qPCR, t test, *p < 0.01. D Verification of the Rv3737 Overexpression Msm strain by Western blotting (full length gels and blots was provided in Additional file 1: Fig. S1 with changes marked)

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