Table 5 Data on inter-and intra-assay variability of the RLEP DRB LAMP formats (in-house and ready-to-use)
From: RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test
Time to positivity (Tp) ( min) | Fluorescence (k) | Annealing temperature (°C) | |||||||
|---|---|---|---|---|---|---|---|---|---|
Mean | SD | CV | Mean | SD | CV | Mean | SD | CV | |
In-house RLEP DRB LAMP | |||||||||
Inter-assay | 14:35 | 02:33 | 0.175 | 29.33 | 4.16 | 0.142 | 92.00 | 0.01 | 0.000 |
Intra-assay | 16:08 | 00:56 | 0.059 | 21.86 | 0.69 | 0.032 | 91.79 | 0.09 | 0.001 |
Ready-to-use RLEP DRB LAMP | |||||||||
Inter-assay | 18:00 | 00:15 | 0.014 | 35.67 | 1.53 | 0.043 | 92.07 | 0.04 | 0.000 |
Intra-assay | 18:04 | 00:24 | 0.022 | 35.71 | 1.80 | 0.050 | 92.04 | 0.07 | 0.001 |