Table 3 Results of the LAMP optimization
From: RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test
Cq value [cycles] | Melting temperature [°C] | |||||
|---|---|---|---|---|---|---|
Mean | SD | CV | Mean | SD | CV | |
Test 1: master mix | ||||||
ISO-DR-001 | 21.78 | 0.13 | 0.006 | 91.90 | 0.00 | 0.000 |
ISO-DR-004 | 14.59 | 0.11 | 0.007 | 91.95 | 0.07 | 0.001 |
Test 2: primer ratio | ||||||
1:1 | 17.05 | 0.01 | 0.001 | 92.00 | 0.00 | 0.000 |
2:1 | 12.77 | 0.51 | 0.040 | 92.00 | 0.00 | 0.000 |
Test 3: annealing temperature (°C) | ||||||
68.6 | 18.64 | 3.42 | 0.183 | 92.20 | 0.28 | 0.003 |
68.1 | 19.21 | – | – | 92.00 | – | – |
67.0 | 19.15 | 2.42 | 0.126 | 92.15 | 0.21 | 0.002 |
65.0 | 15.97 | 0.74 | 0.047 | 92.00 | 0.00 | 0.000 |
62.6 | 19.24 | 0.89 | 0.046 | 91.95 | 0.07 | 0.001 |
60.6 | 21.76 | 0.98 | 0.045 | 92.00 | 0.14 | 0.002 |
59.3 | 24.26 | 0.59 | 0.024 | 91.95 | 0.07 | 0.001 |
58.6 | 28.41 | 1.81 | 0.064 | 91.85 | 0.07 | 0.001 |