Table 2 Number, type and origin of samples used for clinical validation of the RLEP DRB LAMP (n = 150)
From: RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test
Sample category | No | Sample type (No) | Origin (No) |
|---|---|---|---|
RLEP qPCR high-positive samples of MB leprosy patients (five samples additionally confirmed by direct DNA sequencing) | 30 | Nasal swab (24) | Togo, INHa (19) |
Germany, DITMb (5) | |||
Slit skin smear (6) | Togo, INHa (5) | ||
Germany, DITMb (1) | |||
RLEP qPCR medium-positive samples of MB leprosy patients (six samples additionally confirmed by direct DNA sequencing) | 30 | Nasal swab (24) | Togo, INHa (13) |
Germany, DITMb (11) | |||
Slit skin smear (5) | Togo, INHa (4) | ||
Germany, DITMb (1) | |||
Skin biopsy (1) | Germany, DITMb (1) | ||
RLEP qPCR low-positive samples of MB leprosy patients (10 samples additionally confirmed by direct DNA sequencing) | 30 | Nasal swab (26) | Togo, INHa (12) |
Germany, DITMb (14) | |||
Buccal swab (2) | Togo, INHa (2) | ||
Slit skin smear (2) | Togo, INHa (1) | ||
Germany, DITMb (1) | |||
RLEP qPCR negative samples of endemic controls (suspected leprosy cases) | 30 | Nasal swab (13) | Togo, INHa (12) |
Germany, DITMb (1) | |||
Buccal swab (5) | Togo, INHa (5) | ||
Slit skin smear (11) | Togo, INHa (11) | ||
Skin biopsy (1) | Germany, DITMb (1) | ||
RLEP qPCR negative samples of healthy non-exposed controls (DITM laboratory staff) | 30 | Nasal swab (30) | Germany, DITMb (30) |