Table.1 Primers used in nested and real-time PCR
Primers | Â | Sequences | Annealing | Amplified fragment (bp) | Refs |
|---|---|---|---|---|---|
Forward | B1–B22 | 5′-AACGGGCGAGTAGCACCTGAGGAGA-3′ | 62 | 115 | Bretagne,1993 [17] |
Reverse | B1–B23 | 5′-TGGGTCTACGTCGATGGCATGACAAC-3′ |  |  |  |
Forward | SAG-4-OP1 | 5′-TGGACCTACGATTTCAAGAAGGC-3′ | 60 | 187 | Odberg-Ferragut,1996 [18] |
Reverse | SAG-4-OP2 | 5′-GCTGCGAGCTCGACGGGCTCATC-3′ |  |  |  |
Forward | MAG-1-IP1 | 5′-GCATCAGCATGAGACAGAAGA-3′ | 53 | 212 | Contini, 2002 [15] |
Reverse | MAG-1-IP2 | 5′-CCAACTTCGAAACTGATGTCG |  |  |  |