Fig. 2

Sensitivity analysis of the loop mediated isothermal amplification (LAMP) for the detection of P. mirabilis on spiked urine samples. A range of 1.5 10-fold dilutions of pellet suspensions of P. mirabilis U2A 1878 were used. A Eye visualization of the LAMP reaction after coloration with the Sybr Green I dye. B Visualization after migration on an agarose gel of the LAMP products. M: marker, 1: 107 CFU/mL, 2: 106 CFU/mL, 3: 105 CFU/mL, 4:104 CFU/mL, 5: 103 CFU/mL, 6: 102 CFU/mL, 7: 101 CFU/mL, T−, control (DW)