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Table 2 Summary of the characteristics of the anti-CHIKV antibodies obtained from ALTHEA Gold Libraries™

From: Isolation and characterization of high affinity and highly stable anti-Chikungunya virus antibodies using ALTHEA Gold Libraries™

Name Librarya HCDR3 (length)b EC50 scFvc KD (nM) scFv EC50 IgGc KD (nM) IgG Tm (ºC) IgGd Yield IgG
(mg/L)
C7 SL2 8 0.534 17.76 0.0160 0.090 69(78) 59.8
C10 SL1 7 1.349 44.9 0.846 5.060 69(86) 59.4
A11 SL2 8 1.479 49 1.509 8.570 69 44.3
NGS2 SL2 17 ND ND 63.530 423.010 70(81) 7.9
NGS4 SL2 14 ND ND 0.714 4.630 70 9.9
4N12 ND 14 ND ND 0.00243 0.016 61 25.1
4J21 ND 18 ND ND 0.00689 0.046 65 14.1
D9 (5F10) ND ND ND ND 0.01651 0.11 ND ND
  1. ND, not determined
  2. aSL1 and SL2 are included in ALTHEA Gold Libraries™
  3. bThe HCDR3 length was defined using Kabat numbering
  4. cThe half maximal effective concentration (EC50) was determined by quantitative ELISA (see Fig. 3)
  5. dThe melting temperature (Tm) was determined by Protein thermal shift assay (see Additional file 1: Fig. S3)