Isolation and characterization of high affinity and highly stable anti-Chikungunya virus antibodies using ALTHEA Gold Libraries™

Table 2 Summary of the characteristics of the anti-CHIKV antibodies obtained from ALTHEA Gold Libraries™

Name	Library^a	HCDR3 (length)^b	EC₅₀ scFv^c	K_D (nM) scFv	EC₅₀ IgG^c	K_D (nM) IgG	Tm (ºC) IgG^d	Yield IgG (mg/L)
C7	SL2	8	0.534	17.76	0.0160	0.090	69(78)	59.8
C10	SL1	7	1.349	44.9	0.846	5.060	69(86)	59.4
A11	SL2	8	1.479	49	1.509	8.570	69	44.3
NGS2	SL2	17	ND	ND	63.530	423.010	70(81)	7.9
NGS4	SL2	14	ND	ND	0.714	4.630	70	9.9
4N12	ND	14	ND	ND	0.00243	0.016	61	25.1
4J21	ND	18	ND	ND	0.00689	0.046	65	14.1
D9 (5F10)	ND	ND	ND	ND	0.01651	0.11	ND	ND

ND, not determined
^aSL1 and SL2 are included in ALTHEA Gold Libraries™
^bThe HCDR3 length was defined using Kabat numbering
^cThe half maximal effective concentration (EC50) was determined by quantitative ELISA (see Fig. 3)
^dThe melting temperature (Tm) was determined by Protein thermal shift assay (see Additional file 1: Fig. S3)

ISSN: 1471-2334