Fig. 1

Identity and functional analysis of the CHIKV-033 isolate. A Representative electron micrographs of negative staining of CHIKV-033 particles purified by sucrose gradient. Images were captured using the Zeiss Libra 120 transmission electron microscope at 80 kV (Scale bars, 2 μm, 0.5 μm, 100 nm and 50 nm). B SDS-PAGE pattern of purified CHIK-033 virions. MiniProtean Stain Free gels (Biorad) showed the typical pattern of the spike proteins, i.e., E1 at ∼48 kDa, E2 at ∼46 kDa and capsid protein (CP) at ∼30 kDa. Precision Plus Protein Unstained Standards (Biorad) was used like molecular weight marker. C Direct ELISA of the anti-CHIKV 4N12, 4J21 and D9 (5F10) control antibodies. Each experiment was carried out at least three times with independent samples. Representative numbers are presented as average ± standard error of the mean (SEM)