Combining stool and stories: exploring antimicrobial resistance among a longitudinal cohort of international health students

Table 1 PCR primer/probe sequences and additional PCR conditions to identify AMR genes in gut microbiota

Primer/probe	Sequence^a 5′ → 3′	Primer/probe concentration in reaction volume (nM)	Cycling conditions
CTX-M_Fw	ATGTGCAGYACCAGTAARGTKATGGC	500	95 °C, 15 min
CTX-M_Rv	ATCACKCGGRTCGCCNGGRAT	500	40 × 95 °C, 15 s;
CTX-M-1_probe	JOE-CCCGACAGCTGGGAGACGAAACGT-BHQ1	100	58 °C, 20 s
CTX-M-2_probe	6FAM-CAGGTGCTTATCGCTCTCGCTCTGTT-BHQ1	100	72 °C, 30 s
CTX-M-9_probe	JOE-CTGGATCGCACTGAACCTACGCTGA-BHQ1	100
NDM_Fw	ATTAGCCGCTGCATTGAT	400	95 °C, 15 min
NDM_Rv	CATGTCGAGATAGGAAGTG	400	42 × 95 °C, 15 s;
NDM_probe	6FAM- CTG[+ C]CA[+ G]AC[+ A]TT[+ C]GGTGC-BHQ1	200	60 °C, 60 s