From: Clinical and epidemiologic characteristics of inconclusive results in SARS-CoV-2 RT-PCR assays
 | Specimens from confirmed cases (n = 124) | Specimens from screening cases (n = 36) | ||||
---|---|---|---|---|---|---|
sgRNA positive | sgRNA negative | p-value | sgRNA positive | sgRNA negative | p-value | |
Patients (n) | 58 | 66 | Â | 20 | 16 | Â |
Symptom | ||||||
 Fever | 14 (24.1%) | 17 (25.8%) | 1 | 0 (0%) | 0 (0%) | 1 |
 Respiratory symptoms | 9 (15.6%) | 14 (21.2%) | 0.491 | 0 (0%) | 0 (0%) | 1 |
 Asymptomatic | 38 (65.6%) | 43 (65.2%) | 1 | 20 (100%) | 16(100%) | 1 |
Days after symptom onset | ||||||
 Median (Q1–Q3)a | 16 (10 – 19) | 17 (13 – 22) | 0.216 | N/A | N/A |  |
 Min | 1 | 3 |  | N/A | N/A |  |
 Max | 43 | 49 |  | N/A | N/A |  |
Specimens | ||||||
 NP/OP | 34 (58.6%) | 37 (56.1%) | 0.774 | 20 (100%) | 16 (100%) | 1 |
 Sputum | 24 (41.4%) | 29 (43.9%) |  |  |  |  |
Presumptive result | ||||||
 Positive | 23 (39.7%) | 22 (33.3%) | 0.465 | 0 (0%) | 0 (0%) | 1 |
 Negative | 35 (60.3%) | 44 (66.7%) |  | 20 (100%) | 16 (100%) |  |
Each RT-PCR result | ||||||
 Allplex (re-test) | ||||||
  Positive | 7 (12.1%) | 11 (16.7%) | 0.623 | 0 (0%) | 0 (0%) | 0.741 |
  Inconclusive | 38 (65.5%) | 38 (57.6%) |  | 9 (45.0%) | 6 (37.5%) |  |
  Negative | 13 (22.4%) | 17 (25.8%) |  | 11 (55.0%) | 10 (62.5%) |  |
 Real-Q | ||||||
  Positive | 26 (44.8%) | 18 (27.3%) | 0.129 | 0 (0%) | 0 (0%) | 1 |
  Inconclusive | 11 (19.0%) | 15 (22.7%) |  | 2 (10.0%) | 1 (6.3%) |  |
  Negative | 21 (36.2%) | 33 (50.0%) |  | 18 (90.0%) | 15 (93.8%) |  |
 BioCore | ||||||
 Positive | 5 (8.6%) | 5 (7.6%) | 0.704 | 0 (0%) | 0 (0%) | 1 |
  Inconclusive | 20 (34.5%) | 28 (42.4%) |  | 1 (5.0%) | 1 (6.3%) |  |
  Negative | 33 (56.9%) | 33 (50.0%) |  | 19 (95.0%) | 15 (93.8%) |  |
 DiaPlexQ | ||||||
  Positive | 32 (55.2%) | 39 (59.1%) | 0.123 | 0 (0%) | 0 (0%) | 1 |
  Inconclusive | 16 (27.6%) | 9 (13.6%) |  | 0 (0%) | 0 (0%) |  |
  Negative | 10 (17.2%) | 18 (27.3%) |  | 20 (100.0%) | 16 (100.0%) |  |