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Table 5 Results of subgenomic RNA (sgRNA) detection on inconclusive specimens from confirmed cases and screening cases

From: Clinical and epidemiologic characteristics of inconclusive results in SARS-CoV-2 RT-PCR assays

 

Specimens from confirmed cases (n = 124)

Specimens from screening cases (n = 36)

sgRNA positive

sgRNA negative

p-value

sgRNA positive

sgRNA negative

p-value

Patients (n)

58

66

 

20

16

 

Symptom

 Fever

14 (24.1%)

17 (25.8%)

1

0 (0%)

0 (0%)

1

 Respiratory symptoms

9 (15.6%)

14 (21.2%)

0.491

0 (0%)

0 (0%)

1

 Asymptomatic

38 (65.6%)

43 (65.2%)

1

20 (100%)

16(100%)

1

Days after symptom onset

 Median (Q1–Q3)a

16 (10 – 19)

17 (13 – 22)

0.216

N/A

N/A

 

 Min

1

3

 

N/A

N/A

 

 Max

43

49

 

N/A

N/A

 

Specimens

 NP/OP

34 (58.6%)

37 (56.1%)

0.774

20 (100%)

16 (100%)

1

 Sputum

24 (41.4%)

29 (43.9%)

    

Presumptive result

 Positive

23 (39.7%)

22 (33.3%)

0.465

0 (0%)

0 (0%)

1

 Negative

35 (60.3%)

44 (66.7%)

 

20 (100%)

16 (100%)

 

Each RT-PCR result

 Allplex (re-test)

  Positive

7 (12.1%)

11 (16.7%)

0.623

0 (0%)

0 (0%)

0.741

  Inconclusive

38 (65.5%)

38 (57.6%)

 

9 (45.0%)

6 (37.5%)

 

  Negative

13 (22.4%)

17 (25.8%)

 

11 (55.0%)

10 (62.5%)

 

 Real-Q

  Positive

26 (44.8%)

18 (27.3%)

0.129

0 (0%)

0 (0%)

1

  Inconclusive

11 (19.0%)

15 (22.7%)

 

2 (10.0%)

1 (6.3%)

 

  Negative

21 (36.2%)

33 (50.0%)

 

18 (90.0%)

15 (93.8%)

 

 BioCore

 Positive

5 (8.6%)

5 (7.6%)

0.704

0 (0%)

0 (0%)

1

  Inconclusive

20 (34.5%)

28 (42.4%)

 

1 (5.0%)

1 (6.3%)

 

  Negative

33 (56.9%)

33 (50.0%)

 

19 (95.0%)

15 (93.8%)

 

 DiaPlexQ

  Positive

32 (55.2%)

39 (59.1%)

0.123

0 (0%)

0 (0%)

1

  Inconclusive

16 (27.6%)

9 (13.6%)

 

0 (0%)

0 (0%)

 

  Negative

10 (17.2%)

18 (27.3%)

 

20 (100.0%)

16 (100.0%)

 
  1. aThe results are presented as medians with interquartile ranges
  2. NP/OP, nasopharyngeal/oropharyngeal swab specimens