Fig. 4

DENV-2 phylogenetic tree. Phylogenetic relationships among the E protein gene sequences of DENV-2 were resolved using a Maximum Likelihood (ML) tree inferred with IQ-TREE under the GTR + R4 model of nucleotide substitution. Robustness of tree topology was assessed with 1000 bootstrap replicates. Nodes with bootstrap values ≥ 95% are shown. The Sylvatic isolates were used to root the tree. The tree was visualized with the CLC Sequence Viewer 8 program (QIAGEN, USA)