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Table 2 Sequence and molecular mass of extension primer and extension product

From: Development and application of a method to detect 27 respiratory pathogens using multiplex RT-PCR combined with MassARRAY technology

Pathogen species

Extension Primer Sequence

Molecular mass of extension primer

Extended base

Extension Product Sequence

Elongation Product Molecular Mass

M.catarrhalis

TCAACGCCCACATTT

4471.9

G

TCAACGCCCACATTTG

4759.1

HRV

CTTTGAGTCCTCCGGC

4824.1

C

CTTTGAGTCCTCCGGCC

5071.3

MP

AAAGCCACCCTGATCAC

5108.4

C

AAAGCCACCCTGATCACC

5355.5

HCoV

ACGGCTGTGTAAAGA

5234.4

T

ACGGCTGTGTAAAGAT

5561.5

Flu-A

CACGCTGCAGTCCTCGCT

5411.5

C

CACGCTGCAGTCCTCGCTC

5658.7

CP

TTCCTCAACCGAAAGGTC

5443.6

C

TTCCTCAACCGAAAGGTCC

5690.7

Flu-B

AAAGGCCATAGGGAATTG

5596.7

C

AAAGGCCATAGGGAATTGC

5843.9

HMPV

ACGCGGCAGTTTTCAGACA

5812.8

A

ACGCGGCAGTTTTCAGACAA

6084

B. pertussis

CTTCCCGCCCAGACCAAT

5966.9

G

CTTCCCGCCCAGACCAATG

6254.1

H. influenzae

GATGCACTTCCACATTATAT

6051

T

GATGCACTTCCACATTATT

6378.1

H5

GATAAACAGTGGCGAGTTCC

6166

C

GATAAACAGTGGCGAGTTCCC

6413.2

S.pyogenes

CCCCCTCCAGGAGCAACTTGA

6336.1

G

CCCCCTCCAGGAGCAACTTGAG

6623.3

S.pneumoniae

GTATCAGATGAAGCAGGTTTG

6525.3

C

GTATCAGATGAAGCAGGTTTGC

6772.4

PIV4

GGTAGTAATGCCCCTTGCTTAA

6725.4

T

GGTAGTAATGCCCCTTGCTTAAT

7052.5

H1N1

CTGTACAGTCAGTGGTTTCCGT

6732.4

G

CTGTACAGTCAGTGGTTTCCGTG

7019.6

S.agalactiae

CCAGGCCCCCCACGATACTCATG

6914.5

C

CCAGGCCCCCCACGATACTCATGC

7161.7

PIV3

GATCTCTGAGGATACAGATGAAT

7111.7

G

GATCTCTGAGGATACAGATGAATG

7398.9

PIV2

CCCTGTTATTTCTACTCTATCTAT

7194.7

G

CCCTGTTATTTCTACTCTATCTATG

7481.9

ADV

CCCCCAAGTACGTGTCGGTGGCAC

7314.7

G

CCCCCAAGTACGTGTCGGTGGCACG

7602

RSV

CTATTCTCTTCCTAATCTAGACATA

7525.9

G

CTATTCTCTTCCTAATCTAGACATAG

7813.1

H3

CGCTAAATAATGAGATCAGATGCAC

7683

C

CGCTAAATAATGAGATCAGATGCACC

7930.2

L.pneumophila

GTATTTTTAAAATTCTTCCCCAAATC

7854.1

C

GTATTTTTAAAATTCTTCCCCAAATCG

8141.3

PIV1

GGGACGAATACGCATATTGCATCA

8003.2

G

GGGACGAATACGCATATTGCATCAC

8250.4

HEV

AAAGGAAACACGGACA

4941.3

G

AAAGGAAACACGGACAC

5188.4

H7

ATGGACAAACTGTACGA

5227.4

A

ATGGACAAACTGTACGAA

5498.6

H3N2 seasonal

AATCTTCCTGTATGCTCA

5424.5

A

AATCTTCCTGTATGCTCAA

5695.8

HBoV

CTCTATATTGAAGGATCTGCAT

6724.4

C

CTCTATATTGAAGGATCTGCATG

7011.6

GAPDH

ATACGACCAAATCTAAGAGAC

6416.3

G

ATACGACCAAATCTAAGAGACG

6769.6