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Fig. 5 | BMC Infectious Diseases

Fig. 5

From: Identification of novel genes involved in apoptosis of HIV-infected macrophages using unbiased genome-wide screening

Fig. 5

siRNAs of Cdk2, Cox7a2, Cstf2t and Znf484 genes induced apoptosis of HIV-HSA-uninfected bystander MDMs. Seven-day-old MDMs were infected with HIV-HSA for 7 days and then transfected with siRNAs specific for Cdk2, Cox7a2, Cstf2t or Znf484 genes for 72 h. Cells were stained with anti-mouse CD24 antibody (HSA-FITC) and Annexin-V-BV711 antibody. Specific killing of HSA+ (ie HIV-infected) and HSA- (HIV uninfected bystander) cells by siRNAs was quantified by counter staining with Annexin-V-BV711 and flow cytometry analysis. For quantification, both HSA-positive and HSA-negative cells were gated. A Gating strategy for apoptosis of HSA-negative bystander macrophages and HSA-positive macrophages. B Apoptosis of Mock-infected, HIV-HSA-exposed uninfected bystander and HIV-HSA-infected macrophages after siRNA transfection for 72 h. The p-values were calculated using Student’s t-test (n = 4)

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