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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Identification of novel genes involved in apoptosis of HIV-infected macrophages using unbiased genome-wide screening

Fig. 4

siRNAs of Cdk2, Cox7a2, Cstf2t and Znf484 genes selectively induced apoptosis of intensely positive HIV-HSA-infected MDMs. Seven-day-old MDMs were infected with 150 ng p24 of HIV-HSA for 7 days and then transfected with siRNAs specific for Cdk2, Cox7a2, Cstf2t or Znf484 genes for 72 h. Cells were trypsinized, harvested, stained with anti-mouse CD24 antibody (HSA-FITC) and Annexin-V BV711 antibody and apoptosis was quantified by flow cytometry. A Gating the HIV-HSA intensely positive cells. B Apoptosis of HSA intensely positive MDMs following transfection with siRNAs specific for the four genes. The percentage of apoptotic cells of HSA intensely positive was calculated. The p-values were calculated using student’s t-test (n = 4). C Representative histograms from one individual showing apoptosis of HSA intensely positive MDMs induced by siRNA transfection of the 4 identified genes

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