Fig. 1

Infection of U937, THP-1, primary MDMs, and U1 cells with 90K shRNA lentivirus pool. A Differentiated U937 cells B Differentiated THP-1 cells and C Primary MDMs were infected with 90K shRNA lentivirus for 48 h followed by selection with puromycin for another 48 h. D U1 cells infected with lentivirus-eGFP were observed microscopically. E Controlling the infection rate of 90K shRNA Lentivirus Pool. On 90K shRNA lentivirus pool, there is no selection marker for the detection of infection rates. To control the infection rate of 90K shRNA lentivirus pool, undifferentiated U937 cells (0.5 × 10⁶) were infected with various amount of 90K shRNA lentivirus pool or 2 MOI lentivirus-eGFP for 48 h. After puromycin selection of infected cells for another 48 h, trypan blue was employed to track dead cells. When 200 μl 90K shRNA lentivirus pool and 2 MOI lentivirus-eGFP were used to infect undifferentiated U937 cells, similar numbers (approximately 35%) of live cells were counted under microscopy, indicating that the infection rate of 200 μl 90K shRNA lentivirus pool corresponded to 2 MOI