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Fig. 5 | BMC Infectious Diseases

Fig. 5

From: Comparative cytokine profiling identifies common and unique serum cytokine responses in acute chikungunya and dengue virus infection

Fig. 5

In vitro CHIKV and DENV infection of PBMCs induced expression of IL-8, IL-13, and MCP-3, but not IL-4. PBMCs from healthy donors were isolated and infected with CHIKV, DENV-1, -2, -3, -4 (MOI if 1) or mock-infected, and supernatant was collected at 0, 6, 24, 48, and 72 h post infection (HPI). Levels of (A) IL-8, (B) MCP-3, (C) IL-13, and (D) IL-4 in supernatant were measured by cytokine-specific sandwich ELISA. Each dot represents the cytokine concentration of an individual donor. Horizontal black bars indicate the respective group median, which are connected with the continuous black line. ELISA kit detection sensitivities are indicated by the grey dashed line. Statistical significances of time and treatment effects comparing either CHIKV, DENV-1, -2, -3, or-4 infected with mock infected PBMCS were analysed by the two-way repeated measure ANOVA. P values from statistically significant results are shown, while black asterisks represent statistical significance via Bonferroni corrected post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)

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