Fig. 2

CovidNudge PCR amplification curves for a pool of 1 positive and 9 negative samples across all six gene targets (2A = RdRp-IP2, 2B = RdRp-IP4, 2C = n1, 2D = n2, 2E = n3, 2F = e-gene), and with variation of the position of the positive across the 10-pool (2G). Legend: 2A-F. X-axis PCR cycle, Y-axis replicate well threshold. All 6 viral target genes showed amplification in most replicates. 2G. Coloured dots denote how testing the positive sputum sample in a pool of 10 varied when the positive sample altered position in relation to the negatives (from position 1–10)