Table 6 Comparison of LAMP reactions using two different dyes, on DNA extracted using QIAGEN. Values in brackets represent the 95% CI
BURULI Primers | MU 2404 Primers | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Calcein | HNB | Calcein | HNB | |||||||||
+ | – | Total | + | – | Total | + | – | Total | + | – | Total | |
IS2404 (+) | 54 | 8 | 62 | 55 | 7 | 62 | 54 | 8 | 62 | 57 | 5 | 62 |
IS2404 (−) | 1 | 42 | 43 | 2 | 41 | 43 | 4 | 39 | 43 | 4 | 39 | 43 |
Total | 55 | 50 | 105 | 57 | 48 | 105 | 58 | 47 | 105 | 61 | 44 | 105 |
Positivity (%) | 51.4 | 52.4 | 51.4 | 54.3 | ||||||||
Sensitivity (%) | 87.10 (76.15 to 94.26) | 88.71 (78.11 to 95.34) | 87.10 (76.15 to 94.26) | 91.94 (82.17 to 97.33) | ||||||||
Specificity (%) | 97.67 (87.7 to 99.94) | 95.35 (84.19 to 99.43) | 90.70 (77.86 to 97.41) | 90.70 (77.86 to 97.41) | ||||||||
PPV (%) | 98.18 (88.59 to 99.73) | 96.49 (87.63 to 99.07) | 93.10 (84.08 to 97.18) | 93.44 (84.82 to 97.32) | ||||||||
NPV (%) | 84.00 (73.30 to 90.94) | 85.42 (74.40 to 92.19) | 82.98 (71.71 to 90.36) | 88.64 (77.00 to 94.78) | ||||||||
Accuracy (%) | 91.43 (84.35 to 96.01) | 91.43 (84.35% to 96.01) | 88.57 (80.89 to 93.95) | 91.43 (84.35 to 96.01) | ||||||||