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Table 6 Comparison of LAMP reactions using two different dyes, on DNA extracted using QIAGEN. Values in brackets represent the 95% CI

From: Evaluation of different DNA extraction methods and loop-mediated isothermal amplification primers for the detection of Mycobacterium ulcerans in clinical specimens

  BURULI Primers MU 2404 Primers
Calcein HNB Calcein HNB
+ Total + Total + Total + Total
IS2404 (+) 54 8 62 55 7 62 54 8 62 57 5 62
IS2404 (−) 1 42 43 2 41 43 4 39 43 4 39 43
Total 55 50 105 57 48 105 58 47 105 61 44 105
Positivity (%) 51.4 52.4 51.4 54.3
Sensitivity (%) 87.10 (76.15 to 94.26) 88.71 (78.11 to 95.34) 87.10 (76.15 to 94.26) 91.94 (82.17 to 97.33)
Specificity (%) 97.67 (87.7 to 99.94) 95.35 (84.19 to 99.43) 90.70 (77.86 to 97.41) 90.70 (77.86 to 97.41)
PPV (%) 98.18 (88.59 to 99.73) 96.49 (87.63 to 99.07) 93.10 (84.08 to 97.18) 93.44 (84.82 to 97.32)
NPV (%) 84.00 (73.30 to 90.94) 85.42 (74.40 to 92.19) 82.98 (71.71 to 90.36) 88.64 (77.00 to 94.78)
Accuracy (%) 91.43 (84.35 to 96.01) 91.43 (84.35% to 96.01) 88.57 (80.89 to 93.95) 91.43 (84.35 to 96.01)