Fig. 5

Results of RT-PCR analysis of the expression of ALS4 in C. pelliculosa. The gene expression of ALS4 in C. pelliculosa was confirmed using RT-PCR, isolating mRNA samples from 21 isolates of C. pelliculosa. cDNA was synthesized as described in Material and Methods. The endogenous control was ACT1. The CT values were calculated using the 2-ΔΔCt methods