Fig. 2
Phenotype of Aspergillus fumigatus isolates. a A total of 1 × 104 conidia /A. fumigatus isolate were spotted on the PDA plates at 30 °C. Colony morphology was observed after 96 h incubation. b A total of 1 × 104 conidia /A. fumigatus isolate were spotted on the PDA plates at 30 °C. Colony diameters were measured after 72, 96, and 120 h and average diameters were calculated from three independent experiments. Error bars represent standard deviations. *P < 0.001, Unpaired t-test. c A total of 5.0 × 105 conidia /A. fumigatus isolate were incubated in 5 mL of yeast glucose (YG) media at 37 °C for 24 h with shaking at 250 rpm. The precipitates obtained were recovered by filtration, frozen at − 80 °C, lyophilized overnight, and weighed. The average biomass was calculated from three independent experiments. Error bars represent standard deviations. P = 0.0286, Unpaired t-test. d A round-bottomed 96-well plate was inoculated with 100 μL of the conidial suspension at a density of 1 × 105 conidia/mL in a Brian medium and incubated at 37 °C for 24 h. The spent culture medium was removed from each well and the adherent cells were washed three times with distilled water (dH2O). Biofilms were stained with 100 μL of 0.1% (w/v) crystal violet solution and washed and destained with 125 μL of 100% ethanol. The absorbance of the destaining solution was measured at 595 nm. Error bars represent standard deviations. *P < 0.001, Unpaired t-test