Klebsiella spp. cause severe and fatal disease in Mozambican children: antimicrobial resistance profile and molecular characterization

Massinga, Arsénia J.; Garrine, Marcelino; Messa, Augusto; Nobela, Nélio A.; Boisen, Nadia; Massora, Sergio; Cossa, Anélsio; Varo, Rosauro; Sitoe, António; Hurtado, Juan Carlos; Ordi, Jaume; Mucavele, Hélio; Nhampossa, Tacilta; Breiman, Robert F.; Whitney, Cynthia G.; Blau, Dianna M.; Bassat, Quique; Mandomando, Inácio

doi:10.1186/s12879-021-06245-x

Table 1 Primers used for PCR assays

From: Klebsiella spp. cause severe and fatal disease in Mozambican children: antimicrobial resistance profile and molecular characterization

Target	Oligonucleotide sequence (5′–3′)	Annealing temperature (°C)	Expected size (bp)	Reference
bla_CTX-M	F: CGATGTGCAGTACCAGTAA	57	579	[33]
bla_CTX-M	R: TTAGTGACCAGAATCAGCGG	57	579	[33]
bla_SHV	F: ATGCGTTATATTCGCCTGTG	57	841	[34]
bla_SHV	R: TTAGCGTTGCCAGTGCTCG	57	841	[34]
bla_TEM	F:ACGCTCAGTGGAACGAAAAC	57	1150	[33]
bla_TEM	R: ATTCTTGAAGACGAAAGGGC	57	1150	[33]
mrkD	F: CCACCAACTATTCCCTCGAA	57	240	[11]
mrkD	R:ATGGAACCCACATCGACATT	57	240	[11]
rmpA	F: ACTGGGCTACCTCTGCTTCA	57	537	[35]
rmpA	F: CTTGCATGAGCCATCTTTCA	57	537	[35]
wzi-K1	F: GGTGCTCTTTACATCATTGC	57	1282	[35]
wzi-K1	R: GCAATGGCCATTTGCGTTAG	57	1282	[35]
entB	F: ATTTCCTCAACTTCTGGGGC	57	371	[11]
entB	R: AGCATCGGTGGCGGTGGTCA	57	371	[11]
ycfM	F: ATCAGCAGTCGGGTCAGC	57	160	[11]
ycfM	R: CTTCTCCAGCATTCAGCG	57	160	[11]
iutA	F: GGCTGGACATCATGGGAACTGG	57	300	[36]
iutA	R: CGTCGGGAACGGGTAGAATCG	57	300	[36]
fyuA	F: GCGACGGGAAGCGATGATTTA	57	547	[11]
fyuA	R: TAAATGCCAGGTCAGGTCACT	57	547	[11]
hlyA	F: AACAAGGATAAGCACTGTTCTGGCT	63	1177	[11]
hlyA	R: ACCATATAAGCGGTCATTCCCGTCA	63	1177	[11]
traT	F: GGTGTGGTGCGATGAGCACAG	63	290	[36]
traT	R: CACGGTTCAGCCATCCCTGAG	63	290	[36]
hlyC	F: AGGTTCTTGGGCATGTATCCT	55	556	[37]
hlyC	R: TTGCTTTGCAGACTGCAGTGT	55	556	[37]
fimH	F: ATGAACGCCTGGTCCTTTGC	55	688	[11]
fimH	R: GCTGAACGCCTATCCCCTGC	55	688	[11]
pet	F: GGCACAGAATAAAGGGGTGTTT	58	302	[38]
pet	R: CCTCTTGTTTCCACGACATAC	58	302	[38]
piC	F: ACTGGATCTTAAGGCTCAGGAT	58	572	[38]
piC	R: GACTTAATGTCACTGTTCAGCG	58	572	[38]
sat	F: TCAGAAGCTCAGCGAATCATTG	58	932	[39]
sat	R: CCATTATCACCAGTAAAACGCACC	58	932	[39]
sepA	F: GCAGTGGAAATATGATGCGGC	58	794	[38]
sepA	R: TTGTTCAGATCGGAGAAGAACG	58	794	[38]
sigA	F: CCGACTTCTCACTTTCTCCCG	58	430	[39]
sigA	R: CCATCCAGCTGCATAGTGTTTG	58	430	[39]

Back to article page

ISSN: 1471-2334

Contact us

Submission enquiries: bmcinfectiousdiseases@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Infectious Diseases

Contact us