An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis

Table 1 Primers and probes used in this study

Target	Gene	Primer and probe sequences (5′-3′)	Reference
Streptococcus pyogenes M75	emm75	Probe: 56-FAM/TGGAAAAGT/ZEN/GAAAATGATGAGCTTCGGG/3IABkFQ	This study
		F:AGTTACCATATGAAGCACGATACAA
		R:GTTCTTCTAATCTCGTAGTCTTACCT
	speB	Probe: FAM-CGGCGCAGGCGGCTTCAAC-BHQ1	[8]
		F: CTAAACCCTTCAGCTCTTGGTACTG
		R: TTGATGCCTACAACAGCACTTTG
	gyrA	F: CGACTTGTCTGAACGCCAAA	[14]
	gyrA	R: GTCAGCAATCAAGGCCAACA	[14]
Streptococcus pneumoniae	lytA	F: ACGCAATCTAGCAGATGAAGCA	[15]
Streptococcus pneumoniae	lytA	R: TCGTGCGTTTTAATTCCAGCT	[15]
Staphylococcus aureus	gltB	F: CGGGTTAGGTGAATTGATTGTTTTAT	[16]
Staphylococcus aureus	gltB	R: CGCATTTGAGCTGAAGTTG	[16]
Haemophilus influenzae	hpd3	F: GGTTAAATATGCCGATGGTGTTG	[17]
Haemophilus influenzae	hpd3	R: TGCATCTTTACGCACGGTGTA	[17]
Mammalian mitochondrial rRNA	16S mt rRNA	F: CGACCTCGATGTTGGATCAG	[18]
Mammalian mitochondrial rRNA	16S mt rRNA	R: GAACTCAGATCACGTAGGACTTT	[18]

ISSN: 1471-2334