Fig. 1From: An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitisPerformance of the emm75 qPCR. qPCR targeting the emm75 or speB gene was performed on genomic DNA isolated from 10-fold dilutions of S. pyogenes M75. The speB qPCR was used to provide a benchmark for the performance of the emm75 qPCR, using FAM-conjugated probes for both reactions. Each point represents the mean and standard deviation (SD) of duplicate reactionsBack to article page