Table 3 The converted SCAR markers of the L. donovani complex in this study
SCAR marker | Length | Primer sequences 5′-3’ | Annealing temp (°C) |
|---|---|---|---|
1-AD17 | 636 | F: TGGCAAACCCTGTATGAGGAAAACGT R: TTGGCAAACCCTCATAGGTTGC | 61 |
2-A816 | 416 | F: GTGACGTAGGCATGCCAGCAAGGTGG R: GTGACGTAGGGGTGGGGATGAAGAGG | 63 |
3-O13 | 1210 | F: GTCAGAGTCCTCGCGGGGTATTC R: GTCAGAGTCCAGTAGATAGGATCGATGCG | 65 |