Fig. 2

Phylogenetic analysis of the global CVA21 VP1 gene. Phylogenetic tree was reconstructed by the neighbour-joining method based on n = 54 available complete CVA21 VP1 gene sequences (894 bp), equivalent to position 2497–3390 of the prototype strain (GenBank accession no. AF546702). CVA21 strains grouped within Cluster I (East Asia, North America, Australia and Europe) and Cluster II (South Asia, Central America and Central Africa) are indicated by brackets. The reliability of the branching nodes was assessed by bootstrap analysis of 1000 replicates. Bootstrap values (> 70%) are shown at the respective branches. Scale bar represents a genetic distance of 0.05 substitutions/site. GenBank accession number, location and year of isolation are shown for previously reported CVA21 strains. The newly generated sequences in this study are indicated in circle