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Table 2 Summary of all interactions observed between DTG and CRF_02AG IN for the WT and seven mutant structures

From: Interaction analysis of statistically enriched mutations identified in Cameroon recombinant subtype CRF02_AG that can influence the development of Dolutegravir drug resistance mutations

#

RAM’s/Accessory Mutations

CRF_02_AG IN

Hydrogen bonds

Ionic contact

1

WT

4 (Ade21, Gua22, Asp64, Asp116)

2 (MG)

2

T66A

2 (Gua22, Glu152)

2 (MG)

3

T97A

3 (Gua22, Asp116, Glu152)

2 (MG)

4

Q148H

3 (Thy11, Gua22, Glu152)

2 (MG)

5

N155H

5 (Thy11, Gua22, Asp64, Cys65, Glu152)

2 (MG)

6

E157Q

3 (Thy11, Gua22, Glu152)

1 (MG)

7

D232N

2 (Thy11, Gua22)

None

8

R263K

2 (Gua22, Asp116)

2 (MG)

  1. Number outside bracket indicates total number of interactions. Abbreviations used: Ade Adenine, Ala (A) Alanine, Asp (D) Aspartic acid, Cys (C) Cysteine, DTG Dolutegravir, Glu (E) Glutamic acid, Gly (G) Glycine, Gua Guanine, His (H) Histidine, Lys (K) Lysine, MG Magnesium ions, Asn (N) Asparagine, Gln (Q) Glutamine, Arg (R) Arginine, RAM’s Resistance associated mutations, Thr (T) Threonine, Thy Thymidine, WT Wild type. In bold are the two accessory mutations that lost MG interactions crucial for DTG Binding. Three letter codes for IN protein residues and terminal end viral DNA nucleotides after 3′ processing are given
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BMC Infectious Diseases

ISSN: 1471-2334

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