Table 2 Primers and probes used for detection of F. tularensis
From: Francisella tularensis human infections in a village of northwest Iran
Gene target | Primer and probe | Sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|---|
ISFtu2 | ISFtu2-F | TTGGTAGATCAGTTGGTAGGATAACC | 97 |
ISFtu2-R | TGAGTTTTATCCTCTGACAACAATATTTC | ||
ISFtu2-Probe | FAM-AAAATCCATGCTATGACTGATGCTTTAGGTAATCCA-TAMRA | ||
fopA | fopA-F | AACAATGGCACCTAGTAATATTTCTGG | 87 |
fopA-R | CCACCAAAGAACCATGTTAAACC | ||
fopA- Probe | FAM-TGGCAGAGCGGGTACTAACATGATTGGT-TAMRA |