Table 2 Primer sequences and annealing temperatures for quantitative PCR
GENE | PRIMER SEQUENCE | ANNEALING TEMPERATURE (°C) |
|---|---|---|
LDLR forward LDLR reverse | 5′-GAGAGCTTGTGCCGAGATGTG-3′ 5′- CCGCAGTTGTTAGTGCCATCA-3′ | 58 |
SREBP2 forward SREBP2 reverse | 5′- CCTGGGAGACATCGACGAGAT-3′ 5′- TGAATGACCGTTGCACTGAAG-3’ | 54 |
HMGCR forward HMGCR reverse | 5’- TGATTGACCTTTCCAGAGCAAG-3′ 5′- CTAAAATTGCCATTCCACGAGC-3’ | 53 |
ABCA1 forward ABCA1 reverse | 5’-GGAAGAACAGTCATTGGGACAC-3′ 5′- GCTACAAACCCTTTTAGCCAGT-3’ | 58 |