Skip to main content

Table 2 Primer sequences and annealing temperatures for quantitative PCR

From: Hepatic expression of cholesterol regulating genes favour increased circulating low-density lipoprotein in HIV infected patients with gallstone disease: a preliminary study

GENE PRIMER SEQUENCE ANNEALING TEMPERATURE (°C)
LDLR forward
LDLR reverse
5′-GAGAGCTTGTGCCGAGATGTG-3′
5′- CCGCAGTTGTTAGTGCCATCA-3′
58
SREBP2 forward
SREBP2 reverse
5′- CCTGGGAGACATCGACGAGAT-3′
5′- TGAATGACCGTTGCACTGAAG-3’
54
HMGCR forward
HMGCR reverse
5’- TGATTGACCTTTCCAGAGCAAG-3′
5′- CTAAAATTGCCATTCCACGAGC-3’
53
ABCA1 forward
ABCA1 reverse
5’-GGAAGAACAGTCATTGGGACAC-3′
5′- GCTACAAACCCTTTTAGCCAGT-3’
58
\