Institutional outbreak involving multiple clades of IMP-producing Enterobacter cloacae complex sequence type 78 at a cancer center in Tokyo, Japan

Harada, Sohei; Aoki, Kotaro; Ohkushi, Daisuke; Okamoto, Koh; Takehana, Kazumi; Akatsuchi, Tomomi; Ida, Keito; Shoji, Daigo; Ishii, Yoshikazu; Doi, Yohei; Moriya, Kyoji; Hayama, Brian

doi:10.1186/s12879-021-05952-9

Table 3 Molecular and microbiological characteristics of carbapenemase-producing Enterobacterales isolates

From: Institutional outbreak involving multiple clades of IMP-producing Enterobacter cloacae complex sequence type 78 at a cancer center in Tokyo, Japan

Patient No.	Isolate name	Date of isolation	Bacterial species of CPE (ANI)^a	Sequence type	Clade^b	Antimicrobial susceptibility^c							MIC (mg/mL)^d		Antimicrobial resistance genes	Plasmid replicons^e
Patient No.	Isolate name	Date of isolation	Bacterial species of CPE (ANI)^a	Sequence type	Clade^b	TZP	FEP	ATM	LVX	SXT	GEN	AMK	IPM	MEM
1	TUM 14647	Jul. 2014	E. hormaechei	78	A	R	R	R	S	S	S	S	4	4	bla_IMP-1, bla_ACT-5, aac(6′)-IIc, qnrB6, fosA, sul1, tet(B)	IncHI2A/IncHI2
2	TUM 14648	Jul. 2014											2	4
3	TUM 14652	Aug. 2014											4	4
4	TUM 14654	Sep. 2014											4	2
5	TUM 14658	Sep. 2014											4	2
6	TUM 14792	Sep. 2014											4	2
7	TUM 14797	Nov. 2014											4	4
8	TUM 17939	Feb. 2015											4	2		IncHI2A/IncHI2
9	TUM 17940	Apr. 2015				I	R	I	I	R	S	S	> 4	> 4
13	TUM 17944	Jul. 2015				R	R	R	S	S	S	S	4	2
11	TUM 17942	Apr. 2015			B	R	SDD	S	R	R	S	S	4	≤1	bla_IMP-11, bla_TEM-1B, bla_ACT-5, aac(6′)-Ia, qnrS1, fosA, sul1	IncFIB (pHCM2) IncL/M (pMU407)
12	TUM 17943	May 2015			C	R	R	R	S	S	S	S	> 4	4	bla_IMP-1, bla_ACT-5, aac(6′)-IIc, aph(3″)-1b, aph(6)-1d, qnrB6, fosA, sul1, sul2, tet(B)	IncHI2A/IncHI2
14	TUM 17945	Jul. 2015											4	4
15	TUM 17946	Aug. 2015											4	4
10	TUM 17941	Apr. 2015	E. asburiae	24	NA	R	R	S	S	S	S	S	4	4	bla_IMP-1, bla_ACT-1, aac(6′)-IIc, fosA, sul1	IncFII (pECLA)/ IncFIB (pECLA)
16	TUM 17947	Sep. 2015	E. xiangfangensis	1331	NA	R	R	R	S	S	S	S	4	4	bla_IMP-1, bla_ACT-16, aac(6′)-IIc, strA, aph(6)-1d, qnrS1, fosA, sul1, tet(B)	IncHI2A/IncHI2 IncFIB (pECLA)
17	TUM 17948	Oct. 2015	E. hormaechei subsp. steigerwaltii	113	NA	S	R	S	S	S	S	S	4	2	bla_IMP-1, bla_ACT-15, aadA1, aac(6′)-IIc, qnrB6, fosA, sul1	IncHI2A/IncHI2 IncFII (pECLA)/ IncFIB (pECLA)
18	TUM 17949	Apr. 2017	E. hormaechei subsp. steigerwaltii	133	NA	R	R	S	I	S	S	S	2	≤1	bla_IMP-1, bla_ACT-7, aac(6′)-IIc, qnrB6, fosA, sul1, tet(B)	IncHI2A/IncHI2

^a Bacterial species and subspecies confirmed by the comparison of average nucleotide identity of study isolates with those of type strains were presented
^b Clades of ST78 isolates determined by core-genome single nucleotide polymorphism-based analysis were presented
^c Results of antimicrobial susceptibility testing performed with BD Phoenix NMIC/ID-208 panel and interpreted according to CLSI M100-S27 guidelines were shown
^d Minimum inhibitory concentrations determined with BD Phoenix NMIC/ID-208 panel were presented
^e Plasmid replicon was underlined if a transconjugant of the isolates was positive for the replicon by PCR-based replicon typing
ANI average nucleotide identity, NA Not applicable, ST sequence type, TZP piperacillin-tazobactam, CTX cefotaxime, CAZ ceftazidime, FEP cefepime, ATM aztreonam, IPM imipenem, MEM meropenem, LVX levofloxacin, SXT trimethoprim-sulfamethoxazole, GEN gentamicin, AMK amikacin, S Susceptible, SDD Susceptible-Dose Dependent, I Intermediate, R Resistant

Back to article page

ISSN: 1471-2334

Contact us

Submission enquiries: bmcinfectiousdiseases@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Infectious Diseases

Contact us