Fig. 5From: Effect of oxidative stress and calcium deregulation on FAM26F (CALHM6) expression during hepatitis B virus infectionEffect of BAPTA-AM treatment on expressions of IRF3, FAM26F, and IFN-β. The mRNA and proteins were extracted from HepAD38 and HepG2/pHBV1.3 (300 ng) cells 3 and 5 days post HBV induction respectively. GADPH was used as an internal control. Upregulation of IRF3, FAM26F, and IFN-β expression was seen in HBV+ (+BAPTA) cells treated with 5 μM BAPTA for 24 h. Significant differences in the FAM26F expression with p = 0.003, 0.01 and p = 0.02, 0.005 were seen in both HepAD38 and HepG2/pHBV1.3 cell lines, however, the increase in IRF3 and IFN-β expression was not found significant. All the experiments were performed in triplicates (±SD) and the significance was calculated by Student’s t-test (*P < 0.05, **P < 0.01). The X-axis indicates the group without BAPTA treatment as HBV+(−BAPTA) and the BAPTA treated group as HBV+(+ BAPTA), while the Y-axis shows IRF3, FAM26F, and IFN-β expressions in both cell lines. NS: non-significantBack to article page