Fig. 3

Effect of NAC treatment on expressions of IRF3, FAM26F, and IFN-β. The mRNA and proteins were extracted from HepAD38 and HepG2/pHBV1.3 (300 ng) cells 3 and 5 days post HBV induction respectively. GADPH was used as an internal control. Upregulation of IRF3, FAM26F, and IFN-β expression was seen in HBV+ (+NAC) cells treated with 250 μM NAC for 24 h. Significant differences were seen in both HepAD38 and HepG2/pHBV1.3 cell lines. All the experiments were performed in triplicates (±SD) and the significance was calculated by Student’s t-test(*P < 0.05, **P < 0.01). The X-axis indicates the group without NAC treatment as HBV+(−NAC) and the NAC treated group as HBV+(+NAC), while the Y-axis shows the IRF3, FAM26F, and IFN-β expressions in both cell lines