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Table 1 Study outcomes, measurements, and process of collection

From: Assessing the efficacy of two dual-active ingredients long-lasting insecticidal nets for the control of malaria transmitted by pyrethroid-resistant vectors in Benin: study protocol for a three-arm, single-blinded, parallel, cluster-randomized controlled trial

Outcome Measurement Collection
Epidemiological outcomes
 Malaria case incidence Rapid diagnostic test taken when temperature ≥ 37.5 °C and/or history of fever for the past 48 h Active case detection: cohort follow-up
 Malaria infection prevalence Rapid diagnostic test whatever the presence of malaria signs Cross-sectional survey
 Moderate to severe anaemia Haemoglobin (measured by Haemocue), defined as < 10 g/dL and < 8 g/dL, respectively. Active case detection: cohort follow-up Cross-sectional survey
 Temperature Digital infra-red ear thermometer
Temperature and history of fever
Active case detection: cohort follow-up Cross-sectional survey
Entomological outcomes
 Indoor and outdoor Anopheles biting density Human landing catch Entomology monitoring
 Mosquito sporozoite rate Standard CSP-ELISA uses to estimate the EIR. The positive sample will be confirmed with a second ELISA using a heating technique Entomology monitoring
Anopheles species identification An. gambiae, An. coluzzii and An. funestus Taq Man Real time PCR Entomology monitoring
Sentinel site and resistance test
 Resistance intensity WHO cylinder assay, collection of adult Anopheles resting indoor Entomology monitoring
 Frequency of Vgsc mutation Taq Man PCR Entomology monitoring
 Identification and intensity of overexpressed CYP6 genes Screening for CYP6 genes by using Microarrays
Reverse-transcription quantitative PCR (RT-qPCR) using for confirming and monitoring the expression of the cytochrome identified
Resistance activity, unfed 3 days old adult Anopheles from larval collection
  1. Abbreviations: EIR Entomological inoculation rate