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Table 2 Overall number of positive blood cultures obtained for different storage conditions, compared with no delay; and number of BacT/ALERT® bottle sensors that indicated growth prior to incubation

From: Impact of delays to incubation and storage temperature on blood culture results: a multi-centre study

Time (h) Temp (°C) Bottles
(n)
Positive (n)a Positive (%) P valuec Sensor positived
0 NA 90 80 88.9 NA 0/25
6 25 °C 90 85 94.4 0.281 0/25
6 Cool-box 90 81 90.0 1.000 0/25
6 40 °C 90 74 82.2 0.289 0/25
12 25 °C 90 76 84.4 0.511 0/25
12 Cool-box 89b 80 89.9 1.000 0/25
12 40 °C 90 77 85.6 0.656 15/25
24 25 °C 90 82 91.1 0.805 20/25
24 Cool-box 90 71 78.9 0.104 20/25
24 40 °C 90 59 65.6 < 0.001 20/25
6 All 270 240 88.9 1.000 0/75
12 All 269b 233 86.6 0.787 15/75
24 All 270 212 78.5 0.030 60/75
All 25 °C 270 243 90.0 0.841 20/75
All Cool-box 269b 232 86.2 0.694 20/75
All 40 °C 270 210 77.8 0.021 35/75
  1. aFor automated systems this is the number of bottles that flagged positive, and for manual systems this is the number of bottles that were positive on sub-culture; bOne bottle excluded due to contamination; cCompared with no delay; dSensor positive if BacT/ALERT® bottle sensor indicated growth prior to incubation. NA Not applicable
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