Skip to main content

Table 1 Precision of the Deki Reader using laboratory derived specimens

From: Continuous quality monitoring in the field: an evaluation of the performance of the Fio Deki Reader™ for rapid HIV testing in South Africa

Deki Reader Version

RDT brand

Result compared to SOC

bConcordance n (%)

HIV positive

HIV negative

Deki & HCW invalid (true invalid)a

True

False

True

False

Intra-instrument reproducibility (5 specimens read 3 times on the same instrument (n = 3 instruments))

V100 (n = 45)

c First Response® HIV-1-2-0

25

d 1

16

41 (97.6)

Determine™ HIV-1/2 Ag/Ab Combo

29

16

45 (100)

c,g Uni-Gold HIV

24

9

d 1

33 (97.1)

Overall (n = 121c,g)

78

1

25

1

16

119 (98.3)

Intra-instrument reproducibility (5 specimens read 5 times on the same instrument)

V200 (n = 50)

First Response® HIV-1-2-0

25

25

50 (100)

One Step Anti-HIV (1&2)

25

e 1

24

49 (98.0)

HIV 1/2/0 Tri-Line

25

25

50 (100)

Overall (n = 150)

75

1

74

149 (99.3)

Inter-instrument reproducibility (5 specimens read across 3 instruments (3 reads per instrument))

V100 (n = 45)

c First Response® HIV-1-2-0

25

d 1

16

41 (97.6)

Determine™ HIV-1/2 Ag/Ab Combo

29

16

45 (100)

c,g Uni-Gold HIV

24

9

e 1

33 (97.1)

Overall (n = 121c,g)

78

1

25

1

16

119 (98.3)

Inter-instrument reproducibility (10 specimens read across 5 instruments)

V200 (n = 50)

f First Response® HIV-1-2-0

21

25

46 (100)

One Step Anti-HIV (1&2)

25

24

d 1

49 (98.0)

f HIV 1/2/0 Tri-Line

21

25

46 (100)

Overall (n = 142f)

67

0

74

141 (99.2)

  1. aNumbers indicate concordant invalid results from both Deki Reader and HCW. No false invalid specimens were observed
  2. bConcordance includes invalid specimens, but excludes errors and specimens that were not processed
  3. cSpecimens not uploaded to Fionet™ (First Response® HIV 1–2-0 n = 3; Uni-Gold HIV n = 9)
  4. dPresence of artefact
  5. eHCW transcription error
  6. fSpecimens not captured by HCW (First Response® HIV-1-2-0 n = 4; HIV 1/2/0 Tri-Line n = 4)
  7. g Deki Reader error (n = 2)