Fig. 1
From: Comprehensive proteomics investigation of P. vivax-infected human plasma and parasite isolates
Schematic of the proteomics workflow used for the identification of P. vivax parasite proteins in clinical samples. a Whole blood samples were confirmed by RDT and PCR for single infection with P. vivax. Samples were processed by centrifugation to obtain plasma and erythrocyte fractions. b Plasma samples were depleted, quantified, digested and subjected to MS analysis c Erythrocyte fractions were saponin-lysed to isolate parasites from infected RBCs (iRBCs). Protein extraction was carried out using parasite lysis buffer, lysates were fractionated using SDS-PAGE and fractions (4–6 fractions) were digested prior to MS analysis. d A targeted proteomics approach (MRM assays) was standardized to validate unique peptides of most recurring parasite proteins