Fig. 3

The clinical development of S. aureus arthritis in rAAV-LUC/IL2 mice. a, Experimental set up. b, Weight loss. c, Mortality. d, Bacterial clearance in the kidneys. e-g, Serum protein levels of e, TNF, f, IL6 and g, IL5. H, Clinical development of arthritis. i-k, The severity of joint inflammation, or synovitis, and bone erosions was evaluated by making a histological scoring of i, synovitis and j, bone erosions. k, Representative H&E stained sections of inflamed joints on day 3 and 10 after bacterial inoculation. In panels b and d-h, bars show the mean ± standard error of the mean (SEM). In panels i and j, data are shown as median, whiskers = min to max. Statistical calculations were made using the Mann-Whitney U-test. Kaplan-Meier survival plots were prepared and the log-rank test was used for comparison between the two survival curves. *P < 0.05, **P < 0.01. ****P < 0.0001. Scale bars, 100 μm. Abbreviations: B, bone; BM, bone marrow; BD, bone destruction; C, cartilage; E, bone erosions; S, Synovitis